Studies on Neisseria gonorrhoeae cultured in liquid medium.

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Studies on Neisseria gonorrhoeae cultured in liquid medium.

Experiments were carried out to determine the minimum amount of inoculum of gonococci that is needed to produce growth in ANM liquid medium. This was found to be less than had previously been thought. Minimum inocula were used to study penicillin sensitivity in liquid medium. This work showed that growth occurred at higher concentrations of penicillin with larger inocula. Evidence was also obta...

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Prolonged survival of Neisseria gonorrhoeae in a new liquid medium.

Despite substantial improvements in solid culture media for gonococci and the development of liquid media for the short-term growth of the organism, we know of no study of the long-term survival of Neisseria gonorrhoeae in liquid medium. A new liquid medium for N. gonorrhoeae formulated in this laboratory is simple, efficient, and promising. It will support growth and sustain inoculated colony ...

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Dense cultures of Neisseria gonorrhoeae in liquid medium.

Cultivation of Neisseria gonorrhoeae was effected in a conical glass culture vessel surrounded by a constant-temperature water jacket, and with facilities for stirring, aeration, and pH measurement and control. With the use of an aerated peptone-based medium, containing polypropylene glycol to prevent foam build-up, the yields obtained over the pH range from 5.8 to 7.4 were determined. The grea...

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Further studies with a selective medium for cultivating Neisseria gonorrhoeae.

A culture medium that is selective for the gonococcus and the meningococcus has been described recently (Thayer and Martin, 1964). The medium with Polymyxin and Ristocetin added permits isolation of the gonococcus from sites highly contaminated with other bacteria. Overgrowth of gonococcal colonies by the commensal bacterial flora present in rectal, vaginal, and cervical specimens is almost com...

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Staphylococcin on Neisseria gonorrhoeae

Phage group 2 staphylococcal strain UT0002 contains a large 56S virulence plasmid with genes that code for both exfoliative toxin and a specific staphylococcin termed Bac R1. Four penicillinase-producing strains and three penicillinsusceptible strains of Neisseria gonorrhoeae were killed by Bac R1. Aftejr 30 min of growth of the penicillin-resistant TR1 strain in 62.5 arbitrary units of Bac R1 ...

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ژورنال

عنوان ژورنال: Sexually Transmitted Infections

سال: 1978

ISSN: 1368-4973

DOI: 10.1136/sti.54.3.160